DNA Sequencing & Mutation Analysis Facility

 

 

Scientific Officers:    Pradnya Kowtal, In-charge

 

Scientific Assistants: Mahida N

 

 

The DNA sequencing and mutation analysis facility houses a dHPLC (Transgenomic, Wave) machine and an ABI automated DNA sequencer. The dHPLC is being routinely used to study polymorphisms and in mutation screening of genes associated with cancer. A four capillary DNA sequencer takes care of the DNA sequencing needs of scientists on campus as well as from other institutes. The average turnaround time is two working days after receiving samples. Users of DNA sequencing facility are charged minimally per sequencing reaction. Recently the sequencing facility has been used by various groups for sequence analysis of genes involved in cell cycle dysfunction, tumorigenesis and cancer predisposition as well as to confirm the engineered mutations in cloned inserts.